The Kirby-Bauer Test

Since bacteria vary in surface, structure, shape, and dexterity to antibiotics, antimicrobic chemics differ in their effectiveness and spectrum of activity. Scientists and doctors needed a way to effectively let tabu types of pathogens in patients, so they could disperse a drug that would particular(prenominal)ally target the organisms. Kirby-Bauer test, also know as the disk-diffusion method, is the about wide used way of test antimicrobial substances. The effectiveness of a certain chemotherapeutic element is determined by the surface of a zone of curtailment on an inoculated agar plate. A zone of curtailment is the theater of operations where a filter physical composition disk soaked with an antimicrobial agent placed on the agar surface inhibits exploitation of inoculated microorganisms. Based on the size of a zone of inhibition and the relation to a specific chemotherapeutic agent used, organisms atomic number 18 classified as sensitive, intermediate, or kind. We volition find out which chemotherapeutic agents are most effective against which organisms. My initial shot was that gram- validatory bacteria such as Staphylococcus aureus would be susceptible to many chemical substance agents used in the lab, since they drop an outer tissue layer that protects the peptidoglycan cubicle wall. Whereas gram-negative bacteria such as Escherichia coli or genus Pseudomonas aeruginosa would be more resistant than gram-positive bacteria because they subscribe to an outer cell membrane. However, I also hypothesized that polymyxin B efficiency not be as effective to gram positive S.aureus as other chemical agents, because since this agent was typically to engage to Lipid A in the outer cell membrane of gram-negative bacteria, polymyxin B would not be able to disperse the thick peptidoglycan layer of S. aureus. Also, we will be able to vulgarise the gram stain of my groups incomprehensible organism based on the relationship between its sensit ivity to other known organisms sensitivities to incompatible antimicrobial agents.\nOur group obtained four small...